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You will have extra CaCl2 and MgCl2. These solutions can be saved and re-autoclaved for the next time you make competent cells. You can also substitute other media like SOB, 2xYT, etc. .
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Autoclaves are suitable for sterilizing a wide range of materials, but it is crucial to understand which items are compatible and which are not. Let’s explore the materials that can and cannot be treated using an autoclave:By utilizing steam to create a high pressure environment and Maintaining a temperature too high for microbial survival, autoclaves are capable is successfully eliminating pathogens.
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For example, you should not autoclave plastic bottles made from either Low Density Polyethylene (LDPE) or High Density Polyethylene (HDPE). These polymers have a narrow temperature range of between -50 and +80°C in the .
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